Protein Purification Reagents and Kits
Genscript Corporation GITR/TNFRSF18Biotin His A
GITR (glucocorticoid-induced tumor necrosis factor receptor) also known as AITR and TNFRSF18 is a 40 kDa transmembrane glycoprotein that functions in immune regulation GIRT is a receptor for TNFSF18 Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death Mediated NF-kappa-B activation via the TRAF2/NIK pathway
Bruker Daltonics High Capacity Amine Sensor, pack of 12
The High Capacity Amine Sensor by Bruker Scientific LLC, part number 1862615, is a specialized sensor designed for efficient detection of amines. Though this product has been retired, it offered superior performance tailored for various industrial applications. Here are some key features and benefits:
- High capacity for extended use.
- Designed for accuracy in detecting amines.
- Robust construction suitable for demanding environments.
- Pack of 12 sensors for convenient procurement.
- Recommended replacement available for continued service.
Genscript Corporation CD96/TACTILE C110S His Hum
The receptors CD96 and TIGIT are expressed on the surface of T and natural killer (NK) cells and recent studies suggest both play important inhibitory roles in immune function CD96 has been shown to modulate immune cell activity in mice with Cd96-/- mice displaying hypersensitive NK-cell responses to immune challenge and significant tumor resistance The counterbalance between the putative inhibitory CD96 and TIGIT receptors and the activating receptor CD226 offers unique strategies for immuno-oncology drug development
Genscript Corporation Benz-Neburase GMP His-tag
GenScript s offerings are cost-effective with reliable and proven performance for removal of contaminating nucleic acids Furthermore the Benz-Neburase s offerings are produced under stringent quality controls with materials and manufacturing process that are fully traceable allowing for support of IND filing and progression of development stages for various applications
ACROBiosystems Protein, Human ENO1, His Tag, HPLC verified
Enolase 1 (ENO1), also known as alpha-enolase, is a glycolytic enzyme catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate. It is a versatile “moonlighting” protein, functioning as a plasminogen receptor and regulating transcription. This protein is for research use only.
- Synonyms: Enolase 1, ENO1, 2-phosphoglycerate dehydratase, ENO1L1.
- Expressed from *E. coli* cells with a polyhistidine tag at the N-terminus.
- Calculated molecular weight: 49.1 kDa. Migrates as 50–55 kDa on SDS-PAGE.
- Endotoxin level is less than 1.0 EU per μg.
- Purity is >95% by SDS-PAGE and >90% by SEC-HPLC.
- Formulated as a 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH 8.0 with glycerol.
- Store at –70°C or lower; stable for 3 months under sterile conditions.
- Appearance: Lyophilized white powder or clear liquid.
Genscript Corporation MSLN/Mesothelin296-598 His
Mesothelin also known as MSLN is a protein that in humans is encoded by the MSLN gene Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF) Although it has been proposed that mesothelin may be involved in cell adhesion its biological function is not known A knockout mouse line that lacks mesothelin reproduces and develops normally
Genscript Corporation CEACAM-5/CD66e His Cynomolgu
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated
Ubpbio NI-NTA AGAROSE, 5ML
Protein Purification Reagents – Ni-NTA Resin (UBPBio catalog# P3020-5, P3020-25, P3020-100) – Ni-NTA XPure Agarose Resin can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni-NTA XPure Agarose Resin consists of 90µm beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni-NTA is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni-NTA XPure Agarose Resin is highly stable and expand the range of suitable operating conditions.
CUBE BIOTECH PureCube 100 Ni-INDIGO Agarose; 500 ml
500 ml 100 Ni-INDIGO Agarose for His-tag-purification, 50% slurry
CUBE BIOTECH PureCube Co-NTA MagBeads; 1 ml
1ml of Co-NTA MagBead slurry (25%) for Protein Purification of His-tagged proteins
Proteintech Group Inc ChromoTek Spot-Trap Magnetic Agarose, Spot-Tag, Immunoprecipitation, 20 reactions
Spot-Trap Magnetic Agarose is an affinity reagent designed for the immunoprecipitation of Spot-tagged proteins. It utilizes an anti-Spot-Tag VHH/Nanobody coupled to magnetic agarose beads to efficiently isolate fusion proteins from various cell extracts, including those from mammalian, plant, bacterial, yeast, and insect sources.
- Magnetic separation for easy and efficient washing.
- Suitable for IP and Co-IP of Spot-tagged proteins without size limitation.
- Does not introduce contaminating heavy and light antibody chains.
- Optimized for downstream mass spectrometry analysis.
- Consistent batch-to-batch quality.
- Short incubation time of 30 to 60 minutes.
- Specifically binds to the Spot-Tag sequence PDRVRAVSHWSS.
- High binding capacity of 17.5 μg of recombinant Spot-tagged protein (˜30 kDa) per 25 μL bead slurry.
New England Biolabs, Inc. NEBExpress Ni Resin – 25 ml
NEBExpress Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and beta-Mercaptoethanol.
AdipoGen NADE M tuberculosis His
Protein. NAD synthetase Mycobacterium tuberculosis with mutation S444A is fused at the N-terminus to a His-tag. Source E. coli. Endotoxin content N/A. Liquid. In 20mM TRIS-HCl, pH 7.8. Purity >98% SDS-PAGE. NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity ATP deamido-NAD L-glutamine H2O = AMP diphosphate NAD L-glutamate
Sigma Aldrich Fine Chemicals Biosciences His Buffer Kit Cytiva 11-0
His Buffer Kit contains carefully prepared buffer concentrates for binding washing and elution of histidine-tagged proteins according to recommended protocols. Moreover method for optimization of imidazole concentration in the binding buffer is described.
Sino Biological Recombinant Rat CD19 Protein (His Tag)
A DNA sequence encoding the Rat CD19 (NP_001013255.2) (Arg19-Gly287) was expressed with a polyhistidine tag at the C-terminus.